Electrical Properties of Neurospora crassa
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Electrical Properties of Neurospora crassa
The internal potential of Neurospora appears to have two components, one (a) which is reduced by anoxia or abolished by respiratory inhibitors such as azide and 2,4-dinitrophenol, and (b) a fraction that remains in the presence of respiratory inhibitors and is sensitive to the external potassium concentration. Under standard conditions 1 mM azide or dinitrophenol diminishes internal potentials ...
متن کاملProperties of chorismate synthase in Neurospora crassa.
Chorismate synthase was isolated from Neurospora crassa by diethylaminoethylcellulose chromatography, and two peaks of activity were obtained. The enzyme in the first peak had a sedimentation coefficient of ~10 S and appeared to be an unstable, larger molecular form of the enzyme in the second peak, which was -8 S. Both forms of the enzyme required TPNH for activity, and the reactions catalyzed...
متن کاملBiotransformation of Hydrocortisone by Neurospora crassa
The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...
متن کاملPurification and properties of Neurospora crassa laccase.
Extracellular Neurospora laccase (p-diphenol:oxygen oxidoreductase; EC 1.10.3.2) has been purified to apparent homogeneity by classical purification techniques. The enzyme, which consists of mainly one form, has a molecular weight of 64,800 and contains 11% carbohydrate. The ultraviolet, visible, and electron paramagnetic resonance spectra indicate that both type I and type II copper are presen...
متن کاملMOLECULAR ANALYSIS OF THE SULFUR REGULATORY CIRCUIT OF NEUROSPORA CRASSA
The sulfur regulatory circuit of the filamentous fungus, Neurospora crassa, consists of a set of unlinked structural genes which encode sulfur catabolic and two major regulatory genes which govern their expression. The cys-3 regulatory gene encode a transacting regulatory protein which activates the expression of cys-14 and ars, whereas the other regulatory genes Scon-l and Scon-2 appear to...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1965
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.49.1.69